Fluorescence
Automate fluorescence assays in any plate format with Reshape and increase throughput
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Analysis
Solid or suspension medium
GFP and RFP
Combine bright-field and fluorescence image information
Reshaping Fluorescence Assays
Fluorescence form the foundation of various laboratory applications, including enzymatic assays, transformant identification, protein detection via ELISA, HT-screening of drug candidate libraries, and analysis of protein-protein interactions.
The Reshape platform offers endpoint or continues fluorescence reading of up to 10 microtiter plates or 15 petri dishes simultaneously. In combination with customized and automated data processing, Reshape Technology achieves deeper insights of your research and improves throughput.
Other applications
Leaf assays
Get insights on herbicide efficacy, disease development, chemical phytotoxicity, or screen for pathogen inhibition by biocontrol candidates.
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Antagonistic assays
Evaluate inhibitory strain properties and inter-species competition through automated growth detection and quantification using Reshape technology
Seed germination
Automatically analyze complex phenotypes like germination rate, root growth, and time-to-germination with Reshape
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Application Study: Screening for increased recombinant protein titer with 21st BIO
Objective
Identify high producers of recombinant protein using classical mutagenesis in Aspergillus oryzae
Introduction
Classical mutagenesis remains a potent method to generate diverse mutations, especially when working with cell factories. This capability enables identification of numerous and less intuitive combinations of mutations, often unattainable with modern targeted approaches. In this study, UV-mutagenesis was employed to generate a mutant library of Aspergillus oryzae containing an expression cassette for fluorescent protein. Continuous assessment of fluorescence signal and identification of strains with improved protein titer was enabled using the Reshape Imaging System (RIS) in combination with automated image analysis.
Results
Mutant Aspergillus oryzae strains display increased fluorescence. Pools of UV-mutagenized A. oryzae spores were plated on induction medium and development of fluorescence was monitored with Reshape technology. AI-powered image analysis facilitated discrimination between enhanced fluorescence stemming from strain improvement and that from confluent growth of overlapping colonies through time course evaluation (Fig. 1).
Enhanced fluorescent strains identified with Reshape. Replating of selected candidate strains and tailored image analysis allowed identification of colonies displaying enhanced fluorescence. Fungal colonies were detected in bright field and the fluorescence was quantified over time in the corresponding areas (Fig. 2).
Increased fluorescence correlates to improved protein yield. To correlate the level of detected fluorescence with protein yield, culture supernatants of the parental strain and selected strains (E1 and E2) were examined by SDS-PAGE. In comparison to the parental A. oryzae strain, the derivatives E1 and E2 significantly improved yield of the recombinant protein (Fig. 3). These results demonstrate the effectiveness of mutagenesis in combination with Reshape technology to increase protein titers in industrial cell factories.
Conclusion
This study validates UV-mutagenesis combined with Reshape Imaging System and tailored image analysis for identification of enhanced protein yield. The results underscore the effectiveness of this combined approach in boosting protein titers in industrial cell factories, offering promising prospects for further advancements in bioengineering applications.
Personal care
Automate testing and analysis of cosmetics and personal care products to ensure product quality, safety, and efficacy.
Industrials
Enable automated high throughput microbial discovery or do library and enzyme screens with much higher resolution.
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