Reshape Imaging System
Datasheet
PLATE COMPATIBILITY:
90mm petri dishes
Microtiter plates including omnitray and 4/6/8/12/24/48/96-well
Each machine is capable of imaging 10 microtiter plates or 15 petri dishes simultaneously (Only one format of plates in the same job)
RUNTIME AND IMAGING INTERVAL:
From a single image up to 180 days of continuous runtime
60 minutes between images as standard. Depending on plate formats and assay type, this can potentially be reduced to 10 minutes or prolonged to 12 hours.
IMAGE SENSOR:
The Reshape Imaging System uses a 12.3 MP Sony imaging sensor with motorized focus and zoom integrated into the imaging head. The Reshape Imaging System has on-board image processing and temporary storage before images are sent to the Amazon S3 servers. The imaging head is moved around for scanning by a proprietary motion system.
SOFTWARE:
Data storage is subject to additional charges based on usage, please request a quotation based on your specific use case. We are happy to discuss specific assays and compression levels to calculate expected costs. We also offer unlimited data storage plans at fixed costs.Image analysis and quantification is available as a separate subscription service.
INTERFACE:
All data is uploaded to secured Amazon S3 servers and accessible through Reshape Biotech’s web-based cloud interface. From this interface, data can be viewed, annotated and downloaded as desired.
Outputs are given as mp4 files (timelapse videos) with timestamps or as individual frames.
LIGHTING:
All Reshape Imaging Systems are equipped with a proprietary backlight panel which is used for general illumination of transparent or semi-transparent media.
A top-light module is installed as standard as well for illuminating plates from the top. This is usually used for opaque colony formation or opaque media such as yoghurt. The backlight and toplight can be used in conjunction for optimal lighting quality. This toplight module is also used for fluorescent excitation.
Two types of trays are available for holding plates for imaging in black and white materials. The white trays allow diffused backlighting and is suitable for most types of plate imaging. The black trays are optimal for maximizing contrast and minimizing stray light for example when using fluorescence imaging.
FLUORESCENCE:
Qualitative GFP and RFP supported as standard if the fluorescence module is purchased.
Assay results depend heavily on specific fluorescent proteins and promoters. Please discuss specific assays with us so we can help evaluate the fluorescent activity of your specific reporter. Depending on the signal strength, some fluorescent reporters may not be suitable. Filters customized to your application may available (up to 3 simultaneously), but might add additional cost, leadtime and require an application test to determine sensitivity.
Quantification and normalization are expected to be possible but will require additional data processing and reference samples/ladders for the specific proteins. We are happy to discuss specific assays and proteins.
See the dedicated fluorescence datasheet for examples and specifications on the standard filters we use for GFP and RFP.
EXTERNAL DIMENSIONS:
Robot itself: W x D x H: 676 mm x 256 cm x 549 mm
Robot with power cable: W x D x H: 726 mm x 256 cm x 549 mm
90mm petri dishes
Microtiter plates including omnitray and 4/6/8/12/24/48/96-well
Each machine is capable of imaging 10 microtiter plates or 15 petri dishes simultaneously (Only one format of plates in the same job)
RUNTIME AND IMAGING INTERVAL:
From a single image up to 180 days of continuous runtime
60 minutes between images as standard. Depending on plate formats and assay type, this can potentially be reduced to 10 minutes or prolonged to 12 hours.
IMAGE SENSOR:
The Reshape Imaging System uses a 12.3 MP Sony imaging sensor with motorized focus and zoom integrated into the imaging head. The Reshape Imaging System has on-board image processing and temporary storage before images are sent to the Amazon S3 servers. The imaging head is moved around for scanning by a proprietary motion system.
SOFTWARE:
Data storage is subject to additional charges based on usage, please request a quotation based on your specific use case. We are happy to discuss specific assays and compression levels to calculate expected costs. We also offer unlimited data storage plans at fixed costs.Image analysis and quantification is available as a separate subscription service.
INTERFACE:
All data is uploaded to secured Amazon S3 servers and accessible through Reshape Biotech’s web-based cloud interface. From this interface, data can be viewed, annotated and downloaded as desired.
Outputs are given as mp4 files (timelapse videos) with timestamps or as individual frames.
LIGHTING:
All Reshape Imaging Systems are equipped with a proprietary backlight panel which is used for general illumination of transparent or semi-transparent media.
A top-light module is installed as standard as well for illuminating plates from the top. This is usually used for opaque colony formation or opaque media such as yoghurt. The backlight and toplight can be used in conjunction for optimal lighting quality. This toplight module is also used for fluorescent excitation.
Two types of trays are available for holding plates for imaging in black and white materials. The white trays allow diffused backlighting and is suitable for most types of plate imaging. The black trays are optimal for maximizing contrast and minimizing stray light for example when using fluorescence imaging.
FLUORESCENCE:
Qualitative GFP and RFP supported as standard if the fluorescence module is purchased.
Assay results depend heavily on specific fluorescent proteins and promoters. Please discuss specific assays with us so we can help evaluate the fluorescent activity of your specific reporter. Depending on the signal strength, some fluorescent reporters may not be suitable. Filters customized to your application may available (up to 3 simultaneously), but might add additional cost, leadtime and require an application test to determine sensitivity.
Quantification and normalization are expected to be possible but will require additional data processing and reference samples/ladders for the specific proteins. We are happy to discuss specific assays and proteins.
See the dedicated fluorescence datasheet for examples and specifications on the standard filters we use for GFP and RFP.
EXTERNAL DIMENSIONS:
Robot itself: W x D x H: 676 mm x 256 cm x 549 mm
Robot with power cable: W x D x H: 726 mm x 256 cm x 549 mm
WEIGHT:
22 kg
INCUBATOR AND TEMPERATURE RANGES:
The Reshape Imaging System has internal temperature control from +5 degrees celsius above ambient temperature and up to 40 degrees celsius.
Temperature stability and homogeneity across chamber is within +/- 1 degree celsius
Rise time from ambient to 37 degrees celsius is less than 60 minutes.
CLEANING AND STERILITY:
The trays and equipment can easily be cleaned and sterilized with ethanol or soap-based cleaning agents. The interior of the machine can be wiped down while the door is open. The actual imaging head, lens and motion system should NOT be cleaned, and is never in contact or close proximity to any plate.The lids are not removed from the plates during imaging.
POWER AND CONNECTIVITY REQUIREMENTS:
Internet connectivity required for operation. Each Reshape Imaging System unit works on both 220v and 110v outlets. Peak power usage is 150 watts with average power consumption of 20 watts.
DATA SECURITY:
See www.reshapebiotech.com/resources/network-connectivity-guide
SERVICE AGREEMENT:
Reshape Biotech offers service agreements for full coverage of any potential malfunctions and replacement maintenance parts. The service agreement is billed annually.